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Figure 5 | Particle and Fibre Toxicology

Figure 5

From: The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles

Figure 5

IL-1α release by J774 macrophages after particle exposure predicts acute lung inflammation upon particle treatment in mice. Intracellular levels of IL-1α in structural cells (CD45- cells), T (CD5+ cells) and B (B220+ cells) lymphocytes, dendritic cells (F4/80- CD11c+ cells) and alveolar macrophages (F4/80+) freshly purified from (a) untreated lung or (b) 3 hours after silica instillation (crystaline DQ12, 2.5 mg). n = 1 to 6. (c) Release of IL-1α by structural cells (CD45- cells), T (CD5+ cells) and B (B220+ cells) lymphocytes, dendritic cells (F4/80- CD11c+ cells) and alveolar macrophages (F4/80+) purified from lungs 3 hours after silica instillation. n = 1 to 6 (d) Release of IL-1α by fresh primary lung macrophages (CD45+ F4/80+) exposed to silica (overnight). n = 4. (e) Intracellular levels of IL-1α in macrophage (J774), fibroblast (MLg) and epithelial (LA4) cell line. n = 2 to 6. (f) Release of IL-1α by J774 exposed overnight to increasing concentration of DQ12, Vitreous Silica (VS), Stöber silica nanoparticles (Stober) and Aerosil 200. n =3 to 4. (g) Release of IL-1α by J774 exposed overnight to various concentration of MWCNT. n =3 to 4. (h) Number of alveolar total cells 24 hours after particle instillation (1.2 mg) or not. (i) Number of alveolar neutrophils (GR1+ cells) assessed by flow cytometry and (j) expression of the pulmonary neutrophilic marker CXCR2 quantified by qRT-PCR in mice 24 hours after particle instillation or not. Levels of (k) mature IL-1β or (l) IL-1α in BAL fluid collected respectively 24 hours or 1 hour after instillation of particles or not. (m) Correlation between alveolar neutrophils (log GR1+ cells) collected 24 hours after particle instillation and levels of IL-1α in supernatant of particle-exposed J774. Values are means ± SEM of 3 to 6 animals. *p < 0.05, **p < 0.01 and ***p < 0.001 denote significant difference between particle-treated and not animals; ns, denotes no significant difference. P-values are estimated by t-test. Correlation is calculated based on Pearson coefficient.

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