Figure 4

Flow cytometry immunophenotyping of DEP-treated T lymphocytes. Flow cytometry analysis of T cell activation markers (A) and cytokine expression at the single cell level (B) carried out in CD4⁺ and CD8⁺ T lymphocytes from 15 healthy donors after treatment with 30 μg/ml of E4 or E5 particles for 48 h (activation markers) or 72 h (cytokine production). For CD4⁺ and CD8⁺ T lymphocyte subsets, data were expressed as the percentage of each subset within the CD4⁺ or CD8⁺ population considered as 100%. Data are represented as box plots displaying medians, 25^th and 75^th percentiles as boxes, and 10^th and 90^th percentiles as whiskers. *p < 0.05 versus untreated cells.