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Figure 3 | Particle and Fibre Toxicology

Figure 3

From: Cationic nanoparticles directly bind angiotensin-converting enzyme 2 and induce acute lung injury in mice

Figure 3

Ace2 deficiency increases the severity of G5 PAMAM nanoparticle-induced acute lung injury. (A) Survival rates of vehicle- (control) or G5 PAMAM-treated (15 μg/g) wild-type (WT) and Ace2-knockout (ACE2 KO) mice. n = 10 mice per group. **p < 0.01 for the comparison of the WT + G5 group with the ACE2 KO + G5 group (log-rank test). (B) Percent changes in the lung elastance of the vehicle control and PAMAM G5-treated (15 μg/g) WT and ACE2 KO mice at the indicated time points. n = 6 mice per group. **p < 0.01 for the comparison of the WT + G5 group with the ACE2 KO + G5 group at the indicated time points. (ANOVA with Bonferroni post-hoc analysis). (C) PaO2 in the arterial blood of vehicle- (control) or G5 PAMAM-treated (15 μg/g) WT and ACE2 KO mice. n = 4–7 mice per group. (two-tailed t-test). (D) Wet-to-dry weight ratios of the lungs of WT and ACE2 KO mice 10 hrs after intratracheal instillation of vehicle (control) or G5 PAMAM (15 μg/g). n = 4 mice per group. (two-tailed t-test). (E) Representative lung pathologies of WT and ACE2 KO mice 10 hrs after the administration of vehicle (control) or G5 PAMAM (15 μg/g). The numbers of infiltrating cells per microscopic field ± SEM are also shown. n = 100 fields analyzed for three mice for each treatment. (two-tailed t-test). Scale bar = 100 μm. Data are shown as the mean values ± SEM, except the survival curve. *p < 0.05 or **p < 0.01.

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