Table 2 Summary of inflammatory cell counts, LDH, total protein, and inflammatory mediators at 24 h after intratracheal instillation of NPs
From: Response-metrics for acute lung inflammation pattern by cobalt-based nanoparticles
BALF measure | VEHPBS | Co3O4 dose (μg/rat) | CoO dose (μg/rat) | ||||
|---|---|---|---|---|---|---|---|
40 | 100 | 400 | 40 | 100 | 400 | ||
Total cells (× 105) | 10.95 ± 1.42 | 23.60 ± 3.64 | 20.48 ± 5.59 | 45.60 ± 7.71*** | 18.90 ± 2.40 | 30.53 ± 0.39 | 23.25 ± 4.10 |
Macrophages (× 105) | 10.34 ± 1.19 | 23.21 ± 3.55 | 16.67 ± 4.63 | 19.82 ± 3.27 | 17.58 ± 1.81 | 24.46 ± 2.64* | 11.83 ± 3.03 |
Neutrophils (× 105) | 0.49 ± 0.39 | 0.38 ± 0.10 | 3.79 ± 3.13 | 25.65 ± 4.80*** | 1.18 ± 1.06 | 4.91 ± 2.02 | 8.02 ± 1.85 |
Eosinophils (× 105) | 0.07 ± 0.02 | 0.00 ± 0.00 | 0.01 ± 0.01 | 0.09 ± 0.05 | 0.10 ± 0.10 | 1.16 ± 0.76 | 3.37 ± 1.23** |
Lymphocytes (× 105) | 0.05 ± 0.05 | 0.02 ± 0.02 | 0.00 ± 0.00 | 0.05 ± 0.05 | 0.03 ± 0.03 | 0.00 ± 0.00 | 0.04 ± 0.04 |
LDH (fold vs control) | 1.00 ± 0.05 | 1.23 ± 0.10 | 1.62 ± 0.17 | 3.47 ± 0.15*** | 2.35 ± 0.08*** | 5.41 ± 0.22*** | 9.21 ± 0.30*** |
Total protein (μg/mL) | 301 ± 5 | 118 ± 14 | 335 ± 116 | 253 ± 18 | 99 ± 5 | 1104 ± 77* | 3767 ± 667*** |
CINC-3 (pg/mL) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 110.4 ± 39.4** | 0.00 ± 0.00 | 27.3 ± 27.3 | 0.00 ± 0.00 |
IL-6 (pg/mL) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 123.4 ± 43.6 | 982.0 ± 349.4*** |
Eotaxin (pg/mL) | 5.7 ± 1.2 | 4.5 ± 1.5 | 67.4 ± 57.4 | 22.5 ± 10.6 | 4.5 ± 1.8 | 265.7 ± 68.9 | 2963.9 ± 870.7*** |
IL-13 (pg/mL) | 17.7 ± 1.9 | 22.6 ± 4.2 | 28.9 ± 4.5 | 16.9 ± 6.0 | 35.8 ± 4.9 | 49.0 ± 9.7 | 108.5 ± 15.6*** |