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Table 3 Summary of inflammatory cell counts, LDH, total protein, and inflammatory mediators at 24 h after intratracheal instillation of CoCl2

From: Response-metrics for acute lung inflammation pattern by cobalt-based nanoparticles

BALF measure VEHsaline CoCl2 (μg/rat)a
69 (31)b 173 (79) 693 (315)
Total cells (× 105) 13.65 ± 2.63 24.68 ± 5.12 28.35 ± 3.96 18.30 ± 1.54
Macrophages (× 105) 11.84 ± 1.36 14.12 ± 1.81 13.66 ± 0.80 2.92 ± 0.746**
Neutrophils (× 105) 1.76 ± 1.38 10.48 ± 3.72* 13.74 ± 3.77** 11.70 ± 2.75***
Eosinophils (× 105) 0.02 ± 0.02 0.06 ± 0.04 0.95 ± 0.56 3.63 ± 0.81**
Lymphocytes (× 105) 0.05 ± 0.01 0.10 ± 0.04 0.04 ± 0.02 0.02 ± 0.02
LDH (fold vs control) 1.00 ± 0.12 3.08 ± 0.53* 6.65 ± 0.78*** 15.05 ± 0.18***
Total protein (μg/mL) 270 ± 47 341 ± 67 700 ± 165 5051 ± 917***
CINC-3 (pg/mL) 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
IL-6 (pg/mL) 0.00 ± 0.00 0.00 ± 0.00 39.2 ± 39.2 734.6 ± 152.3***
Eotaxin (pg/mL) 10.76 ± 3.27 30.94 ± 16.60 133.1 ± 49.2 699.8 ± 38.1***
IL-13 (pg/mL) 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
  1. aCo ions were prepared by dissolving CoCl2 in saline and treated at equivalent dose for Co of CoO NPs (40, 100, and 400 μg/rat)
  2. bParenthesis represents the Co ions mass for CoCl2 which is equivalent Co ions doses of CoO NPs
  3. Data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001, statistically different from vehicle control (VEHsaline)
  4. VEHsaline, sterile 0.9 % saline was used as a vehicle control for CoCl2; LDH, lactate dehydrogenase; CINC-3, cytokine-induced neutrophil chemoattractant-3