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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Translocation of gold nanoparticles across the lung epithelial tissue barrier: Combining in vitro and in silico methods to substitute in vivo experiments

Fig. 5

Translocation kinetics of AuNP through A549 and MLE-12 CMLs. (a) Translocation fraction of 18 nm AuNP after 24 h for different doses. (b) Translocation fraction 24 h after exposure to 100 ng/cm2 AuNP for different sizes. (c) Translocation fraction of 18 nm AuNP at a dose of 100 ng/cm2 after different times post-exposure. The in vitro results were compared to in vivo data from Kreyling et al. [20] and Schleh et al. [30]. Kreyling et al. determined the translocation fraction of AuNP of various sizes after 24 h (b) and of 18 nm AuNP after various time points (c) in female Wistar-Kyoto rats after intratracheal instillation. Schleh et al. determined the translocation fraction of 20 nm large AuNP in female C57BL/6 mice following two hours of inhalation exposure (c). (detailed information on properties and dose of the AuNP used in the in vivo studies may be found in Additional file 1 (Table S1); data are expressed as mean ± SD, n = 3 (CMLs, # n = 2), n = 4 (rats/mice) and the yellow and blue areas show the uncertainty range using Monte Carlo analysis (PBPK model, n = 1000 iterations); significant differences between A549 and MLE-12 CMLs: * p < 0.05, ** p < 0.01)

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