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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells

Fig. 2

Induction of reactive oxygen species by 50 nm UNP, CNP and ANP in the absence and presence of antioxidant N-acetylcysteine. Cells were exposed to 25 μg/ml of 50 nm NPs for 4 h, alone (a-d, i-l, q-t) and in the presence (e-h, m-p, u-x) of 10 mM NAC. All types of NPs induced significant ROS production in AT2 (i-l) and MAC (q-t), whereas TT1 cells (a-d) were most susceptible to ANP. NAC treatment significantly prevented NP-induced ROS in epithelial cells (aa, e-h, ab, m-p), but not macrophages (ac, u-x). ROS were measured in live cells and data are presented as mean fluorescence intensity (MFI) ± SD (n = 3 TT1 replicates and 6 subject samples for AT2 and MAC) in aa-ac *p < 0.05; **p < 0.001

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