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Table 4 Oligonucleotides used for PCR amplification. The gene name, the sequence of forward (Fw) and reverse (Rv) oligonucleotide, the PCR product and the Genbank ID are shown

From: Acute and subchronic exposure to air particulate matter induces expression of angiotensin and bradykinin-related genes in the lungs and heart: Angiotensin-II type-I receptor as a molecular target of particulate matter exposure

Gen Olinucleotides PCR product (pb) Genbank ID
At1r Fw 5'-AATATTTGGAAACAGCTTGGT-3' 331 [GenBank: NM_030985]
Rv 5'-ATGATGATGCAGGTGACTTTG-3'
At2r Fw 5'-TAGTTCCCCTTGTTTGGTG-3' 428 [GenBank: NM_012494]
Rv 5'-GAGGATGGCAAAAGGAAGT-3'
Ace Fw 5'-CCAACAAGACTGCCACCTG-3' 457 [GenBank: NM_012544]
Rv 5'-GTACTGGTGACATCGAGGTTG-3'
B1r Fw 5'-AGCATCTTCCTGGTGGTGG-3' 420 [GenBank: NM_030851]
Rv 5'-CCAGCAGACCAGGAAGGAG-3'
B2r Fw 5'-GAGATCTACCTGGGCAACCT-3' 599 [GenBank: NM_173100]
Rv 5'-AGGAAGGTGCTGATCTGGAA-3'
Klk-1 Fw 5'-CCCTCACCCTGACTTCAAC-3' 236 [GenBank: 001005382]
Rv 5'-TCACACACTGGAGCTCATC-3'
Acta1 Fw 5'-ACATCGACATCAGGAAGGAC-3' 234 [GenBank: NM_019212]
Rv 5'-CGTCGTACTCCTGCTTGGT-3'
Col3a1 Fw 5'-AGGGTGATCGTGGTGAAAA-3' 239 [GenBank: NM_032085]
Rv 5'-TCCTCGATGTCCTTTGATG-3'
18S Fw 5'-GCAGCTAGGAATAATGGAATA-3' 188 [GenBank: NR_046237]
Rv 5'-GACTTTCGTTCTTGATTAATGA-3'
Gapdh Fw 5'-ACCACAGTCCATGCCATCAC-3' 166 [GenBank: NM_017008]
Rv 5'-TGCCAGTGAGCTTCCCGTT-3'