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Table 12 Results of the comet assay with and without hOGG1 in the lung, kidneys and liver of male rats 24 h after a 90-day exposure to Graphistrength© C100

From: Lung inflammation and lack of genotoxicity in the comet and micronucleus assays of industrial multiwalled carbon nanotubes Graphistrength© C100 after a 90-day nose-only inhalation exposure of rats

Test groups (n = 5/group)

hOGG1

Air control

Graphistrength© C100 (mg/m3)

Positive controla

  

0

0.05

0.25

5.0

 

LUNG

% of DNA in tailb

-

8.8 ± 5.5

3.4 ± 1.5*

8.2 ± 5.1

4.7 ± 3.7

48.7 ± 3.0**

+

20.2 ± 5.2

16.7 ± 5.7

22.3 ± 11.8

17.9 ± 5.9

79.1 ± 7.9**

Relative ratio of ghost cellc

-

-

1.0

1.2

0.8

0.6**

+

-

2.1**

2.0**

1.3

1.1

KIDNEYS

% of DNA in tailb

-

7.4 ± 4.4

7.5 ± 4.2

8.2 ± 4.2

9.7 ± 3.1

72.5 ± 5.8**

+

22.8 ± 7.4

22.4 ± 7.3

23.4 ± 8.4

24.3 ± 8.0

74.0 ± 5.9**

Relative ratio of ghost cellc

-

-

1.6

0.9

1.5

2.8**

+

-

1.1

1.6*

1.7**

0.6*

LIVER

% of DNA in tailb

-

3.8 ± 3.1

3.4 ± 1.6

1.8 ± 1.0

5.5 ± 2.9

71.2 ± 9.1**

+

11.6 ± 6.4

7.5 ± 1.5

7.7 ± 1.1

9.6 ± 3.0

78.6 ± 3.7**

Relative ratio of ghost cellc

-

-

0.2

0.5

2.8**

4.9**

+

-

0.5*

0.2**

1.1

1.7**

  1. */**/***: Non-parametric Mann-Whitney test significant <0.05 (*), <0.01 (**)
  2. aMMS
  3. bMean of median ± sd
  4. cMean value in treated groups/mean control value