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Table 12 Results of the comet assay with and without hOGG1 in the lung, kidneys and liver of male rats 24 h after a 90-day exposure to Graphistrength© C100

From: Lung inflammation and lack of genotoxicity in the comet and micronucleus assays of industrial multiwalled carbon nanotubes Graphistrength© C100 after a 90-day nose-only inhalation exposure of rats

Test groups (n = 5/group) hOGG1 Air control Graphistrength© C100 (mg/m3) Positive controla
   0 0.05 0.25 5.0  
LUNG
% of DNA in tailb - 8.8 ± 5.5 3.4 ± 1.5* 8.2 ± 5.1 4.7 ± 3.7 48.7 ± 3.0**
+ 20.2 ± 5.2 16.7 ± 5.7 22.3 ± 11.8 17.9 ± 5.9 79.1 ± 7.9**
Relative ratio of ghost cellc - - 1.0 1.2 0.8 0.6**
+ - 2.1** 2.0** 1.3 1.1
KIDNEYS
% of DNA in tailb - 7.4 ± 4.4 7.5 ± 4.2 8.2 ± 4.2 9.7 ± 3.1 72.5 ± 5.8**
+ 22.8 ± 7.4 22.4 ± 7.3 23.4 ± 8.4 24.3 ± 8.0 74.0 ± 5.9**
Relative ratio of ghost cellc - - 1.6 0.9 1.5 2.8**
+ - 1.1 1.6* 1.7** 0.6*
LIVER
% of DNA in tailb - 3.8 ± 3.1 3.4 ± 1.6 1.8 ± 1.0 5.5 ± 2.9 71.2 ± 9.1**
+ 11.6 ± 6.4 7.5 ± 1.5 7.7 ± 1.1 9.6 ± 3.0 78.6 ± 3.7**
Relative ratio of ghost cellc - - 0.2 0.5 2.8** 4.9**
+ - 0.5* 0.2** 1.1 1.7**
  1. */**/***: Non-parametric Mann-Whitney test significant <0.05 (*), <0.01 (**)
  2. aMMS
  3. bMean of median ± sd
  4. cMean value in treated groups/mean control value