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Table 1 Physical and chemical characterization of Ag ENMs

From: Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating

  Anionic Ag ENMs Neutral Ag ENMs Cationic Ag ENMs
Name Ag_Citrate Ag_SDS Ag_Disperbyk Ag_Tween Ag_Byk Ag_Chitosan
Stabilizer   Sodium citrate 0.025 % SDS 0.05 % Disperbyk 192® 0.05 % Tween 80® 0.1 % BYK 9076® 0.025 % in 0.05 % acetic acid Chitosan 0.05 % in 0.1 % acetic acid
Surface charge [mV]a In solvent - 42.26 ± 1.64 −33.23 ± 0.90 - 3.02 ± 4.12 −1.13 ± 1.73 +26.56 ± 1.67 +52.40 ± 1.79
In RPMI 1600 Medium −8.43 ± 0.84 −9.8 ± 1 .56 −3,38 ± 1.27 −1.2 ± 1.36 −9.8 ± 0.89 −4,83 ± 0.3
Surface chemistry [Atom %] C 44.6; O 41.6; Na 9.2; N 3.0; Ag 1.7 C 48.1; O 40.2; Na 5.9; Ag 3.6; N 2.0; S 0.2 C 72.8; O 24.5; Ag 1.6; N 1.0 C 71.5; O 25.7; Na 2.6; Ag 0.2 C 69.5; O 16.3; N 9.4; Ag 4.7 C 46.3; O 41.3; N 7.3; Na 3.4; Ag 1.7
Size/size distribution & aggregation/agglomeration state [nm]a In solvent DLS: 5.4 DLS: 5.0 DLS: 9.1 DLS: 8.0 DLS: 9.2 DLS: −
TEM: 5.9 ± 2.3 TEM: 6.2 ± 2.9 TEM: 6.9 ± 2.8 TEM: 6.1 ± 2.1 TEM: 10.5 ± 2.5 TEM: 9.8 ± 2.1
XRD: 6.3 XRD: 6.8 XRD: 5.9 XRD: 6.3 XRD: 6.8 XRD: 7.1
In RPMI 1600 medium DLS: DLS: DLS: DLS: DLS: DLS:
P1: 133 ± 67.56 P1: 137.4 ± 78.24 P1: 391.5 ± 10.9 P1: 49.73 ± 10.9 P1: 34.83 ± 2.54 P1: 763.7 ± 379.4
P2 : 22.04 ± 9.2 P2: 17.14 ± 5.31 P2: 41.18 ± 10.3    P2: 127.4 ± 37.93
Shape spherical spherical spherical spherical spherical spherical
Crystal structure cubic Cubic cubic cubic cubic cubic
Concentration [μg/ml] 100 100 100 100 100 100
  1. aexpressed as mean ± SD of 3 independent replicates