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Table 2 Concentrations of Ag ENMs (A) and Ag ENM stabilizers (B) applied for different assay. Ag ENM concentrations are expressed in mass units [per volume/per exposure area/per cell] and in area of ENMs [per volume/per exposure area/per cell]. Stabilizer concentrations are expressed in % w/v

From: Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating

  A: Ag ENMs concentrations
Mass units Surface area of ENMs
[μg/ml] [μg/cm2] [pg/cell] [cm2/ml] [cm2/cm2] [cm2/cell]
CA RGA TBE CM 1 0.31 ˜6.67 0.72 0.22 ˜ 4.79×10−6
2 0.63 ˜ 13.33 1.44 0.45 ˜ 9.59×10−6
4 1.25 ˜ 26.67 2.88 0.9 ˜ 1.92×10−5
8 2.5 ˜ 53.33 5.75 1.8 ˜ 3.87×10−5
10 3.13 ˜ 66.67 7.19 2.25 ˜ 4.84×10−5
GMA 2 0.63 ˜ 19.73 1.44 0.45 ˜ 1.42×10−5
4 1.25 ˜ 39.47 2.88 0.9 ˜ 2.84×10−5
8 2.5 ˜ 78.93 5.75 1.8 ˜ 5.68×10−5
B: Ag ENM stabilizers concentrations
  Citrate SDS Disperbyk Tween Byk Chitosan
TBE 0.0025 0.005 0.005 0.01 0.0025 0.005
RGA 0.0025 0.005 0.005 0.01 0.0025 0.005
CM 0.002 0.005 0.005 0.01 0.0025 0.005
CA 0.002 0.004 0.004 0.008 0.002 0.004
GMA 0.002 0.004 0.004 0.008 0.001 0.002
  1. TBE Trypan Blue exclusion assay, RGA Relative Growth activity, CM cells morphology, CA Comet assay, GMA Gene mutation assay