Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating

Table 2 Concentrations of Ag ENMs (A) and Ag ENM stabilizers (B) applied for different assay. Ag ENM concentrations are expressed in mass units [per volume/per exposure area/per cell] and in area of ENMs [per volume/per exposure area/per cell]. Stabilizer concentrations are expressed in % w/v

	A: Ag ENMs concentrations
	Mass units			Surface area of ENMs
	[μg/ml]	[μg/cm²]	[pg/cell]	[cm²/ml]	[cm²/cm²]	[cm²/cell]
CA RGA TBE CM	1	0.31	˜6.67	0.72	0.22	˜ 4.79×10⁻⁶
	2	0.63	˜ 13.33	1.44	0.45	˜ 9.59×10⁻⁶
	4	1.25	˜ 26.67	2.88	0.9	˜ 1.92×10⁻⁵
	8	2.5	˜ 53.33	5.75	1.8	˜ 3.87×10⁻⁵
	10	3.13	˜ 66.67	7.19	2.25	˜ 4.84×10⁻⁵
GMA	2	0.63	˜ 19.73	1.44	0.45	˜ 1.42×10⁻⁵
	4	1.25	˜ 39.47	2.88	0.9	˜ 2.84×10⁻⁵
	8	2.5	˜ 78.93	5.75	1.8	˜ 5.68×10⁻⁵
B: Ag ENM stabilizers concentrations
	Citrate	SDS	Disperbyk	Tween	Byk	Chitosan
TBE	0.0025	0.005	0.005	0.01	0.0025	0.005
RGA	0.0025	0.005	0.005	0.01	0.0025	0.005
CM	0.002	0.005	0.005	0.01	0.0025	0.005
CA	0.002	0.004	0.004	0.008	0.002	0.004
GMA	0.002	0.004	0.004	0.008	0.001	0.002

TBE Trypan Blue exclusion assay, RGA Relative Growth activity, CM cells morphology, CA Comet assay, GMA Gene mutation assay

ISSN: 1743-8977