Table 3 Concentrations of Ag ions in Ag ENM samples and Ag ions released after incubation in cell culture media were evaluated by ICP-MS. Ag ions in Ag ENM stock were expressed as % of total Ag in Ag ENM samples. Ag ions were isolated from Ag ENMs by (a) extraction and (b) by ultracentrifugation. Ag ions released during 24 h incubation in cell culture medium were quantified by CP-MS and expressed as percentage of the total added Ag ENMs. Results are presented as mean ± standard deviation of 3–6 replicates
Ag ions (%) in stock | Released Ag ions after 24 h incubation in cell culture medium | ||
|---|---|---|---|
Obtained by extraction | Obtained by ultracentrifugation | ||
Ag_Citrate | 12.16 ± 5.19 | 8.41 ± 0.64 | 7.02 ± 3.27 |
Ag_SDS | 13.81 ± 2.49 | 7.28 ± 2.56 | 6.44 ± 1.93 |
Ag_Disperbyk | 25.72 ± 4.33 | 15.86 ± 0.49 | 5.25 ± 1.37 |
Ag_Tween | 19.12 ± 1.93 | 7.72 ± 1.45 | 6.68 ± 1.87 |
Ag_Chitosan | x | 14.37 ± 9.6 | 11.34 ± 6.37 |
Ag_Byk | x | 13.59 ± 5.63 | 3.94 ± 0.04 |