Skip to main content

Advertisement

Table 3 Concentrations of Ag ions in Ag ENM samples and Ag ions released after incubation in cell culture media were evaluated by ICP-MS. Ag ions in Ag ENM stock were expressed as % of total Ag in Ag ENM samples. Ag ions were isolated from Ag ENMs by (a) extraction and (b) by ultracentrifugation. Ag ions released during 24 h incubation in cell culture medium were quantified by CP-MS and expressed as percentage of the total added Ag ENMs. Results are presented as mean ± standard deviation of 3–6 replicates

From: Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating

  Ag ions (%) in stock Released Ag ions after 24 h incubation in cell culture medium
Obtained by extraction Obtained by ultracentrifugation
Ag_Citrate 12.16 ± 5.19 8.41 ± 0.64 7.02 ± 3.27
Ag_SDS 13.81 ± 2.49 7.28 ± 2.56 6.44 ± 1.93
Ag_Disperbyk 25.72 ± 4.33 15.86 ± 0.49 5.25 ± 1.37
Ag_Tween 19.12 ± 1.93 7.72 ± 1.45 6.68 ± 1.87
Ag_Chitosan x 14.37 ± 9.6 11.34 ± 6.37
Ag_Byk x 13.59 ± 5.63 3.94 ± 0.04
  1. x – Ag ion concentrations cannot be determined for the positively charged Ag ENMs using this approach