Fig. 1

The experimental study design and the number of animals per each evaluated endpoint. All mice (dams and non-pregnant mice) were exposed to Cu NPs or filtered laboratory air (4 hrs/day) from gestation day (GD) 3 to 19. A selected number of mice was euthanized on GD 19 and the pups were delivered by Caesarian section (0 wk post exposure). Another group of mice delivered the pups spontaneously on GD 18–20, these pups grew until adolescence when they were euthanized at 50 postnatal days (PND). ^# There were 4 mice in the pregnant group exposed to Cu NPs, all other experimental groups had 5 animals