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Table 2 NP oxidative stress potential

From: Proteomic analysis of protein carbonylation: a useful tool to unravel nanoparticle toxicity mechanisms

   Cytotoxicity Surface reactivity NP (NP free supernatant) ROS – Activity Protein carbonylation
   IC50 (WST) ESR ESR ESR DCFDA-Assay 1D screen 2D test
   μg/ml (μg/cm2) CPH/ dH2O DMPO/dH2O Overall μg/ml (μg/cm2) Highest treatment μg/mL (μg/cm2) Relative Spot number
SiO2 unmod. weak (IC 50 not reached) 4 (0.88) 11 (6.3) Positive n.d. 34 (10) Strong 74
PEG no cytotoxicity 1 (3.4) 11 (13) Negative n.d. 34 (10) Negative N.A.
Amino no cytotoxicity 0.57 (1.1) 21 (5.2) Positive n.d. 34 (10) Medium 78
Phosphate no cytotoxicity 2.2 (1.2) 19 (5) Positive n.d. 34 (10) Weak 78
NM-200 no cytotoxicity 3.7 1.0 Positive n.d. 170 (50) Medium 86
NM-203 week (IC 50 not reached) 3.3 1.1 Positive n.d. 170 (50) Medium 121
ZrO2 Acrylate no cytotoxicity 1 (2) 3.6 (1.5) Negative n.d. 34 (10) Negative N.A.
PEG no cytotoxicity 1.5 (2.3) 1.7 (0.71) Negative n.d. 34 (10) Negative N.A.
Amino no cytotoxicity 0.95 (17) 3.5 (2) Negative n.d. 34 (10) Negative N.A.
TODS no cytotoxicity 0.54 (5.7) 0.94 (1.3) Negative n.d. 34 (10) Negative N.A.
Ag 50 PVP 1 (0.3) 45 (1) 0.4 (1) Positive n.d. 34 (10) Strong 93
200 PVP no cytotoxicity 72 (1) 0.48 (1) Positive n.d. 34 (10) Negative N.A.
50 Citrate no cytotoxicity N.A. N.A. N.A. n.d. 34 (10) Medium 44
NM-300 k 12 (3.6) 1.4 (4.3) 0.5 (1.3) Negative n.d. 34 (10) Medium 62
TiO2 NM-103 no cytotoxicity 3.2 1.0 Positive n.d. 85 (25) Negative N.A.
NM-104 no cytotoxicity 1.6 0.9 Negative n.d. 85 (25) Negative N.A.
NM-105 Strong 0.82 3 Positive n.d. 34 (10) Medium 57
MWCNT NM-400 no cytotoxicity 2.1 1.2 Negative 34 (10)a 170 (50) Negative N.A.
NM-401 no cytotoxicity 2.4 0.8 Negative 170 (50)a 170 (50) Negative N.A.
NM-402 no cytotoxicity 1.1 3.4 Positive 34a (10) 170 (50) Negative N.A.
ZnO NM-111 20 (6) 2.8 3.5 Positive n.d. 170 (50) Strong 88
NM-110 (stir)b 7.5 (2.2) 22 12 Positive n.d. 170 (50) Strong 68
NM-110 (US)b 12.5 (3.7) 3.4 2.8 Positive n.d. 170 (50) Strong 80
BaSO4 NM-220 no cytotoxicity 2 2 Negative N.A. N.A. Negative N.A.
AlOOH no cytotoxicity 2.3 1.3 Negative N.A. N.A. Negative N.A.
  1. Values in brackets for ESR refer to NP-depleted supernatant, if applicable. Protein carbonylation strong, medium, and weak was assigned according to 1D immunoblots.
  2. Data labeled with a) are derived from Farcal et al. [71]
  3. brefers to dispersion method used (stir: stirring or US: ultrasonication)