Fig. 1
From: Assessing particle and fiber toxicology in the respiratory system: the stereology toolbox
Lung sampling for stereology. a Lung fixation under defined inflation pressure. b Assessment of the lung volume or reference space by the Archimedes’ principle. c Serial sections of the lung – optionally with isotropic uniform random (IUR) orientation with the orientator. d Systematic uniform random sampling (SURS) of lung sections: in this example, every 3rd section is included in the sampling. The first section is picked at random - either by throwing a dice or with a random number table. e If smaller samples are desired for tissue embedding a further SUR sub-sampling is performed. Again every third tissue piece is selected here and the 1st one chosen randomly. f Selected tissue blocks are embedded for LM or TEM – optionally with IUR orientation using the isector