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Table 4 List of primary monoclonal antibodies are used for IHC staining

From: Morphometric analysis of inflammation in bronchial biopsies following exposure to inhaled diesel exhaust and allergen challenge in atopic subjects

Antibody

Marker

Cell

Concentration (μg.mL−1)

Catalog no.

Source

AA1

Tryptase

Mast cells

0.1

ab2378

Abcam Inc., Toronto, ON

EG2 (614)

ECP

Eosinophils

0.07

514-121

Diagnostics, Uppsala, SW

IL-4 (4D9)

IL-4

Th2, mast cells

20.0

211-44-134AX

Amsbio, Cambridge, UK

CD4 (4B12)

CD4

Helper T cells

8.0

M731001-2

Dako, Burlington, ON

CD138 (B-A38)

Syndecan-1

Plasma cells

1.0

MCA2459GA

AbD Serotec, Raleigh, NC

NE (NP57)

Elastase 2

Neutrophils

0.1

M075201-2

Dako, Burlington, ON