Skip to main content
Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation

Fig. 4

Time course of 8-nitroG formation and iNOS expression in MWCNT-treated cells. Immunofluorescent images of 8-nitroG formation (a) and iNOS expression (b) in MWCNT-treated A549 cells. A549 cells were treated with 1 μg/ml MWCNT for indicated durations at 37 °C, and 8-nitroG formation and iNOS expression were examined by fluorescent immunocytochemistry as described in Methods. Hoechst, Hoechst 33258. Magnification, X200. (c, d) Relative staining intensity of 8-nitroG and iNOS in A549 cells. Staining intensities of 8-nitroG (c) and iNOS (d) per cell were analyzed by an ImageJ software. Relative staining intensity of the control at 4 h was set at 1. Data represent means ± SD of 4 (control) and 6 (CNT-S and CNT-L) independent experiments. **p < 0.01 and ***p < 0.001, compared with the control; # p < 0.05 and ## p < 0.01, compared with CNT-S-treated cells by ANOVA followed by Tukey’s test

Back to article page