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Fig. 9 | Particle and Fibre Toxicology

Fig. 9

From: Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation

Fig. 9

Association of TLR9 with HMGB1 and RAGE in MWCNT-treated cells. A549 cells were treated with 1 μg/mL MWCNT for 8 h. Double immunofluorescent analysis was performed to examine the colocalization of TLR9 with HMGB1 (a) and RAGE (b) as described in Methods. HMGB1 and RAGE were stained with the corresponding mouse monoclonal antibodies and Alexa 488-labeled goat anti-mouse IgG antibody. TLR9 was stained with the rabbit polyclonal antibody and Alexa 594-labeled goat anti-rabbit IgG antibody. Hoechst, Hoechst 33258. Magnification, X400. Arrows indicate the colocalization of TLR9 with HMGB1 or RAGE in the cytosol. c Interaction of TLR9 with RAGE and HMGB1. Immunoprecipitation (IP) was performed using anti-TLR9 rabbit polyclonal antibody and TLR9, RAGE and HMGB1 were detected by Western immunoblotting (IB). The membrane was treated with corresponding mouse monoclonal antibodies. Data are representative of 3 independent experiments

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Particle and Fibre Toxicology

ISSN: 1743-8977

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