Eye irritation testing of nanomaterials using the EpiOcular™ eye irritation test and the bovine corneal opacity and permeability assay

Table 7 Mean opacity and permeability values and corresponding in vitro irritation score (IVIS) of aSiO₂-susp, quartz dust DQ12, talc, and three organic pigments determined in the BCOP assay

Test material	aSiO₂-susp	Quartz dust DQ12	Pigment Red 57:1	Pigment Yellow 95	Pigment Black 32	Talc
Volume or mass applied	750 μL	120 mg	48 mg	45 mg	40 mg	80 mg
Mean opacity value ± SD	14.9 ± 4.7	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
Mean permeability value ± SD	0.04 ± 0.02	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0
IVIS ± SD	15.4 ± 4.5	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0

All substances were applied undiluted using the BCOP treatment protocol for solids (4 h test substance exposure without further post-incubation)
Abbreviations: IVIS In vitro irritation score, NC negative control, PC positive control, SD standard deviation

ISSN: 1743-8977