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What level of throughput can be achieved?
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VITROCELL® 6/6
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VITROCELL® Cloud 6
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Cultex® RFS
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NACIVT
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• 6-well inserts (can use adaptors to adapt to 12 or 24-well-sized)
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• 6-well inserts
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• 6, 12, or 24-well inserts
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• 6-well inserts
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• compartments for exposing 6 cell culture inserts
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• compartments for exposing 6 cell culture inserts: 6 inserts at one exposure; no clean air control
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• compartments for exposing 3 cell culture inserts - all exposed to the same substance
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• compartments for exposing 24 cell culture inserts – allexposed to the same substance.
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• allows for separate clean air exposure in one module:
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• 1 well can be used for the (optional) microbalance.
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• would have to purchase 2 modules for clean air control
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• would have to purchase 2 modules for clean air control
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o 3 replicates clean air control
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o 3 replicates of one dose (or 2 replicates plus optional 1 well for microbalance)
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VITROCELL® 12/12
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VITROCELL® Cloud 12
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Cultex® RFS Compact
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• 12-well inserts (can use adaptors to adapt to 24-well-sized)
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• 12-well inserts
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• 12 or 24-well inserts
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• compartments for exposing 12 cell culture inserts
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• compartments for exposing 12 cell culture inserts
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• compartments for exposing 6 cell culture inserts
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• allows for separate clean air exposure in one module:
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• allows for separate clean air exposure in one module:
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• allows for separate clean air exposure in one module
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o 3 replicates clean air control
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o 3 replicates for clean air control
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o 3 dose dilutions with 3 replicates per dilution (or 2 replicates plus optional 1 well for microbalance)
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o 9 inserts at one exposure (1 well can be used for the optional microbalance)
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VITROCELL® 24/24
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VITROCELL® Cloud 24
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• 24-well inserts
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• 24-well inserts
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• compartments for exposing 24 cell culture inserts
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• compartments for exposing 24 cell culture inserts; 24 inserts at one exposure (1 well can be used for the optional microbalance)
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• allows for separate clean air exposure in one module:
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• would have to purchase 2 modules for clean air control
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o 4 replicates clean air control
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o 5 dose dilutions with 4 replicates per dilution (or 3 replicates plus optional 1 well for microbalance)
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Commercially available?
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Yes
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Yes
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Yes
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No
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Deposited-dose determination
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• The microbalance is capable of measuring the deposition in the module at a resolution of 10 ng/cm2.
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1 well can be used for the microbalance (optional).
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Gravimetric methods, using the precision balance.
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One of the wells is designed to carry a transmission electron microscope (TEM) grid to monitor exposure. Also has an electrometer.
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• For the 6/6 and 12/12, a built-in microbalance option is available. A microbalance sensor would occupy one compartment; therefore, the module should have at least 4 compartments in order to allow 3 replicates for exposure.
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• For the 24/24, the purchase of a separate, standalone 12/1 CF module to use a microbalance would be needed.
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Exposure Method
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The VITROCELL® 6/6 is compatible with electrostatic deposition.
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Not compatible with electrostatic deposition, but due to high deposition efficiency of NMs in the Cloud, electrostatic deposition is not needed (Cloud is equipped with a nebulizer that generates liquid aerosols).
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Compatible with electrostatic deposition (both RFS and RFS compact).
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Compatible with electrostatic deposition.
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The 12/12 and 24/24 are not, but in those cases, the VITROCELL® 6/6 modules can be used with adaptors for 12 or 24-well inserts.
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Exposure time duration
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Maximum 6 h possible
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Approximately 3–4 min
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Up to 1 h reported
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Up to 2 h reported
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Ability to utilize multiple aerosol generating techniques?
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May be used with a wide range of aerosol generators.
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Compatible only with the aerosol generator, which is part of the delivery (vibrating mesh type for liquid aerosols).
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May be used with a wide range of aerosol generators; One option is the Cultex® dust generator, which is a Mitchell or screw mill type.
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May be used with a wide range of aerosol generators; Can be placed close to the workplace or aside a busy street.
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