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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Atomic layer deposition coating of carbon nanotubes with zinc oxide causes acute phase immune responses in human monocytes in vitro and in mice after pulmonary exposure

Fig. 3

Cytokine mRNA expression in THP-1 cells 24 h after exposure to Z-MWCNTs, U-MWCNTs or ZnO NPs. a Taqman qRT-PCR was used to quantify IL-6, IL-1β, CXCL10 and TNF-α mRNA levels. Significant increases in pro-inflammatory cytokines were observed for both Z-MWCNT and ZnO NP treated cells. Asterisks represent comparisons to the control (*P < 0.05, **P < 0.01, and ***P < 0.001). Asterisks above a bar represent comparison to U-MWCNT. b Representative TEM images of THP-1 cells (upper panel) and U-MWCNT within cytoplasm (Cyt) of a THP-1 cell (lower panel). ‘Nu’ denotes nucleus. Arrows indicate U-MWCNTs within THP-1 cells. Z-MWCNTs were not observed within THP-1 cells

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