Fig. 6

Histopathologic findings in the liver. a: typical microscopic pattern of the mouse liver – the control group. b-h: CNT exposed groups. b: 1 day after instillation (a.i.) to 54 μg: hypertrophy of Kupffer cells (long arrows), numerous binucleate hepatocytes (short arrows); c: 28 days a.i. to 54 μg: − granuloma surrounded by eosinophilic necrotic hepatocytes (long arrow), vacuolar degeneration (short arrows); d: 28 days a.i. to 54 μg: − macrophages (arrows), parenchymatous degeneration; e, f: 1 day after instillation (a.i.) to 162 μg: macrophages (long arrows), vacuolar degeneration (short arrows); g: 3 days a.i. to 162 μg: pronounced vacuolar degeneration in the central zone of the liver lobule, numerous binucleate hepatocytes (arrows); h: 28 days a.i. to 162 μg: foci of necrosis (asterisks), small granuloma (head of arrow), hypertrophy of Kupffer cells (long arrows), oedema (short arrows). i-j: The EPOXY-REF exposed groups, dose 486 μg i – 3 day a.i.: small degree vacuolar degeneration of hepatocytes; j – 28 days a.i.: typical pattern of the liver. k-n: The EPOXY-CNT exposed groups. k - 3 days a.i. to 162 μg: hyperplasia of Kupffer cells (arrows); l - 28 days a.i. to 162 μg: vacuolar degeneration on the whole area of the liver lobule, hyperplasia of the bile ducts epithelium (arrows); m - 28 days a.i. to 162 μg: mild-zonal vacuolar degeneration; n - 3 days a.i.to 486 μg: vacuolar degeneration of hepatocytes on the whole area of the liver lobule. Staining HE, magnification on the figures a – e and g - m as scale on the figure (n). o-u: The EPOCYL exposed groups. o - 1 day a.i. to 54 μg: hypertrophy of Kupffer cells (arrows); p – 28 days a.i. to 54 μg: vacuolar degeneration on the whole area of the liver lobule, hyperplasia of the bile ducts epithelium (arrows); r - 1 day a.i. to 162 μg: vacuolar degeneration on the whole area of the liver lobule, hypertrophy of Kupffer cells (arrows); s – 3 days a.i. to 162 μg: mild-zonal vacuolar degeneration of hepatocytes; t - 3 days a.i. to 486 μg: vacuolar degeneration of hepatocytes on the whole area of the liver lobule, granuloma surrounded by eosinophilic necrotic hepatocytes (arrow), u: same pattern as on the figure (t) in double magnification. Staining HE, magnification on the figures (o – s) as scale on the figure (t)