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Table 3 BAL fluid counts in mice 1, 3 and 28 days post exposure to 54 μg, 162 μg and 486 μg sanding dust from epoxy and 162 μg CB

From: Epoxy composite dusts with and without carbon nanotubes cause similar pulmonary responses, but differences in liver histology in mice following pulmonary deposition

   Control   EPOXY-REF    EPOXY-CNT    EPOCYL   CBa
1 day 0 μg 54 μg 162 μg 486 μg 54 μg 162 μg 486 μg 54 μg 162 μg 486 μg 162 μg
  Neutrophils (x103) 9.4 ± 2.5 37.8 ± 5.0** 110.1 ± 20.8*** 189.3 ± 16.2*** 72.7 ± 8.8*** 154.4 ± 20.1*** 182.3 ± 22.2*** 42.0 ± 7.8** 149.7 ± 12.1*** 188.3 ± 15.2*** 143.7 ± 26.1***
  Macrophages (x103) 62.8 ± 5.9 93.5 ± 14.7 72.3 ± 16.7 49.3 ± 6.8 62.5 ± 5.1 70.6 ± 13.1 53.4 ± 2.9 85.8 ± 9.5 65.3 ± 11.6 41.4 ± 7.0 18.4 ± 4.5**
  Eosinophils (x103) 1.0 ± 0.5 19.4 ± 3.7*** 51.3 ± 8.7*** 28.1 ± 5.0*** 48.4 ± 9.4*** 91.5 ± 13.7*** 17.4 ± 8.5* 17.6 ± 5.3** 58.8 ± 7.9*** 16.0 ± 7.0** 20.2 ± 5.7**
  Lymphocytes (x103) 0.8 ± 0.2 2.8 ± 1.0 3.6 ± 1.0 4.6 ± 1.2* 5.2 ± 1.9 6.6 ± 2.7 6.8 ± 1.3* 2.1 ± 0.6 3.7 ± 1.3 3.5 ± 1.2 0.9 ± 0.5
  Epithelial (x103) 8.8 ± 1.6 9.0 ± 1.7 10.7 ± 3.5 13.7 ± 4.2 9.7 ± 2.5 10.8 ± 3.1 13.1 ± 2.8 9.7 ± 2.2 10.6 ± 2.7 13.4 ± 1.7 9.8 ± 2.1
  Total BAL cells (x103) 82.8 ± 8.3 162.4 ± 21.0** 248.0 ± 38.2*** 285.0 ± 28.4*** 198.5 ± 11.1*** 334.0 ± 29.1*** 273.0 ± 32.7*** 157.2 ± 15.4** 288.0 ± 22.2*** 262.5 ± 19.9*** 193 ± 26.4**
3 days            
  Neutrophils (x103) 2.6 ± 1.2 5.0 ± 1.3 40.9 ± 7.3*** 94.5 ± 19.7*** 28.0 ± 8.4*** 26.2 ± 4.8*** 113.5 ± 27.3*** 10.5 ± 1.3** 50.6 ± 8.6*** 151.5 ± 17.6*** 120.2 ± 24.4***
  Macrophages (x103) 57.8 ± 5.8 81.0 ± 12.5 96.9 ± 10.6 111.2 ± 22.2 78.0 ± 13.2 95.9 ± 16.4 125.1 ± 23.5* 84.8 ± 8.6 146.9 ± 11.8*** 98.6 ± 32.4 65.5 ± 16.3
  Eosinophils (x103) 5.3 ± 4.4 36.8 ± 5.8*** 99.6 ± 22.8*** 30.3 ± 12.9* 95.4 ± 14.0*** 139.5 ± 36.1*** 32.8 ± 9.0* 56.5 ± 22.1*** 173.0 ± 47.7*** 41.8 ± 9.5*** 43.8 ± 16.3
  Lymphocytes (x103) 1.7 ± 0.6 2.8 ± 0.6 29.6 ± 7.1*** 53.7 ± 24.2*** 10.6 ± 2.9* 34.2 ± 5.7*** 35.5 ± 21.9 9.3 ± 3.5** 25.5 ± 5.4*** 14.0 ± 3.5*** 3.1 ± 1.4
  Epithelial (x103) 7.1 ± 1.5 7.0 ± 0.7 9.5 ± 1.7 21.9 ± 2.4** 8.5 ± 2.6 10.3 ± 3.0 14.1 ± 3.4 11.9 ± 2.5 14.4 ± 2.6 24.6 ± 2.8** 12.8 ± 4.5
  Total BAL cells (x103) 74.5 ± 8.8 132.5 ± 17.2 276.5 ± 34.3*** 311.5 ± 64.4*** 220.5 ± 33.4*** 306.0 ± 46.1*** 321.0 ± 67.9*** 173.0 ± 30.8*** 410.5 ± 52.0*** 330.5 ± 31.6*** 254.4 ± 28.3***
28 days            
  Neutrophils (x103) 6.8 ± 4.2 3.4 ± 1.4 8.8 ± 3.2 29.6 ± 5.4** 6.9 ± 1.7 18.2 ± 4.1* 30.2 ± 5.0*** 5.3 ± 2.1 12.0 ± 2.4 53.8 ± 9.8*** 59.3 ± 8.6***
  Macrophages (x103) 60.3 ± 5.7 53.1 ± 11.1 74.6 ± 8.4 100.2 ± 18.3 67.9 ± 12.7 93.1 ± 11.2 99.3 ± 11.9 50.9 ± 5.5 75.0 ± 5.9 84.2 ± 10.1 118.2 ± 29.5
  Eosinophils (x103) 20.4 ± 10.1 18.5 ± 8.1 3.2 ± 0.7 3.2 ± 0.8 8.8 ± 2.7 6.3 ± 3.4 2.2 ± 1.0 10.0 ± 3.6 2.2 ± 0.9 2.5 ± 0.7 1.7 ± 1.0
  Lymphocytes (x103) 4.9 ± 1.6 6.2 ± 2.1 5.7 ± 1.3 7.9 ± 1.6 4.7 ± 1.4 6.3 ± 0.8 10.1 ± 3.1 10.7 ± 4.0 6.4 ± 1.1 8.6 ± 1.3 36.4 ± 12.6
  Epithelial (x103) 9.8 ± 1.9 11.8 ± 3.5 7.2 ± 2.6 13.5 ± 2.2 7.8 ± 2.6 7.6 ± 1.8 14.6 ± 2.7 10.1 ± 3.9 11.0 ± 2.1 12.9 ± 2.8 18.0 ± 3.0
  Total BAL cells (x103) 102.3 ± 16.3 93.0 ± 19.2 99.5 ± 11.8 154.5 ± 18.1** 96.0 ± 17.0 131.5 ± 15.5 156.5 ± 12.8 87.0 ± 10.4 106.5 ± 7.5 162.0 ± 16.5* 233.5 ± 23.7**
  1. Mean ± SEM
  2. *p < 0.05 compared to controls, **p < 0.01 compared to controls, ***p < 0.001 compared to controls
  3. There were no statistically significant differences between the three sanding dusts at the 0.05 level
  4. aOne mouse in the 162 μg CB group on day 3 post-exposure was considered an outlier because the total number of BAL cells was 10 times higher than the rest of the group. Therefore the BAL results from this mouse were excluded from the BAL dataset