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Table 3 BAL fluid counts in mice 1, 3 and 28 days post exposure to 54 μg, 162 μg and 486 μg sanding dust from epoxy and 162 μg CB

From: Epoxy composite dusts with and without carbon nanotubes cause similar pulmonary responses, but differences in liver histology in mice following pulmonary deposition

  

Control

 

EPOXY-REF

  

EPOXY-CNT

  

EPOCYL

 

CBa

1 day

0 μg

54 μg

162 μg

486 μg

54 μg

162 μg

486 μg

54 μg

162 μg

486 μg

162 μg

 

Neutrophils (x103)

9.4 ± 2.5

37.8 ± 5.0**

110.1 ± 20.8***

189.3 ± 16.2***

72.7 ± 8.8***

154.4 ± 20.1***

182.3 ± 22.2***

42.0 ± 7.8**

149.7 ± 12.1***

188.3 ± 15.2***

143.7 ± 26.1***

 

Macrophages (x103)

62.8 ± 5.9

93.5 ± 14.7

72.3 ± 16.7

49.3 ± 6.8

62.5 ± 5.1

70.6 ± 13.1

53.4 ± 2.9

85.8 ± 9.5

65.3 ± 11.6

41.4 ± 7.0

18.4 ± 4.5**

 

Eosinophils (x103)

1.0 ± 0.5

19.4 ± 3.7***

51.3 ± 8.7***

28.1 ± 5.0***

48.4 ± 9.4***

91.5 ± 13.7***

17.4 ± 8.5*

17.6 ± 5.3**

58.8 ± 7.9***

16.0 ± 7.0**

20.2 ± 5.7**

 

Lymphocytes (x103)

0.8 ± 0.2

2.8 ± 1.0

3.6 ± 1.0

4.6 ± 1.2*

5.2 ± 1.9

6.6 ± 2.7

6.8 ± 1.3*

2.1 ± 0.6

3.7 ± 1.3

3.5 ± 1.2

0.9 ± 0.5

 

Epithelial (x103)

8.8 ± 1.6

9.0 ± 1.7

10.7 ± 3.5

13.7 ± 4.2

9.7 ± 2.5

10.8 ± 3.1

13.1 ± 2.8

9.7 ± 2.2

10.6 ± 2.7

13.4 ± 1.7

9.8 ± 2.1

 

Total BAL cells (x103)

82.8 ± 8.3

162.4 ± 21.0**

248.0 ± 38.2***

285.0 ± 28.4***

198.5 ± 11.1***

334.0 ± 29.1***

273.0 ± 32.7***

157.2 ± 15.4**

288.0 ± 22.2***

262.5 ± 19.9***

193 ± 26.4**

3 days

           
 

Neutrophils (x103)

2.6 ± 1.2

5.0 ± 1.3

40.9 ± 7.3***

94.5 ± 19.7***

28.0 ± 8.4***

26.2 ± 4.8***

113.5 ± 27.3***

10.5 ± 1.3**

50.6 ± 8.6***

151.5 ± 17.6***

120.2 ± 24.4***

 

Macrophages (x103)

57.8 ± 5.8

81.0 ± 12.5

96.9 ± 10.6

111.2 ± 22.2

78.0 ± 13.2

95.9 ± 16.4

125.1 ± 23.5*

84.8 ± 8.6

146.9 ± 11.8***

98.6 ± 32.4

65.5 ± 16.3

 

Eosinophils (x103)

5.3 ± 4.4

36.8 ± 5.8***

99.6 ± 22.8***

30.3 ± 12.9*

95.4 ± 14.0***

139.5 ± 36.1***

32.8 ± 9.0*

56.5 ± 22.1***

173.0 ± 47.7***

41.8 ± 9.5***

43.8 ± 16.3

 

Lymphocytes (x103)

1.7 ± 0.6

2.8 ± 0.6

29.6 ± 7.1***

53.7 ± 24.2***

10.6 ± 2.9*

34.2 ± 5.7***

35.5 ± 21.9

9.3 ± 3.5**

25.5 ± 5.4***

14.0 ± 3.5***

3.1 ± 1.4

 

Epithelial (x103)

7.1 ± 1.5

7.0 ± 0.7

9.5 ± 1.7

21.9 ± 2.4**

8.5 ± 2.6

10.3 ± 3.0

14.1 ± 3.4

11.9 ± 2.5

14.4 ± 2.6

24.6 ± 2.8**

12.8 ± 4.5

 

Total BAL cells (x103)

74.5 ± 8.8

132.5 ± 17.2

276.5 ± 34.3***

311.5 ± 64.4***

220.5 ± 33.4***

306.0 ± 46.1***

321.0 ± 67.9***

173.0 ± 30.8***

410.5 ± 52.0***

330.5 ± 31.6***

254.4 ± 28.3***

28 days

           
 

Neutrophils (x103)

6.8 ± 4.2

3.4 ± 1.4

8.8 ± 3.2

29.6 ± 5.4**

6.9 ± 1.7

18.2 ± 4.1*

30.2 ± 5.0***

5.3 ± 2.1

12.0 ± 2.4

53.8 ± 9.8***

59.3 ± 8.6***

 

Macrophages (x103)

60.3 ± 5.7

53.1 ± 11.1

74.6 ± 8.4

100.2 ± 18.3

67.9 ± 12.7

93.1 ± 11.2

99.3 ± 11.9

50.9 ± 5.5

75.0 ± 5.9

84.2 ± 10.1

118.2 ± 29.5

 

Eosinophils (x103)

20.4 ± 10.1

18.5 ± 8.1

3.2 ± 0.7

3.2 ± 0.8

8.8 ± 2.7

6.3 ± 3.4

2.2 ± 1.0

10.0 ± 3.6

2.2 ± 0.9

2.5 ± 0.7

1.7 ± 1.0

 

Lymphocytes (x103)

4.9 ± 1.6

6.2 ± 2.1

5.7 ± 1.3

7.9 ± 1.6

4.7 ± 1.4

6.3 ± 0.8

10.1 ± 3.1

10.7 ± 4.0

6.4 ± 1.1

8.6 ± 1.3

36.4 ± 12.6

 

Epithelial (x103)

9.8 ± 1.9

11.8 ± 3.5

7.2 ± 2.6

13.5 ± 2.2

7.8 ± 2.6

7.6 ± 1.8

14.6 ± 2.7

10.1 ± 3.9

11.0 ± 2.1

12.9 ± 2.8

18.0 ± 3.0

 

Total BAL cells (x103)

102.3 ± 16.3

93.0 ± 19.2

99.5 ± 11.8

154.5 ± 18.1**

96.0 ± 17.0

131.5 ± 15.5

156.5 ± 12.8

87.0 ± 10.4

106.5 ± 7.5

162.0 ± 16.5*

233.5 ± 23.7**

  1. Mean ± SEM
  2. *p < 0.05 compared to controls, **p < 0.01 compared to controls, ***p < 0.001 compared to controls
  3. There were no statistically significant differences between the three sanding dusts at the 0.05 level
  4. aOne mouse in the 162 μg CB group on day 3 post-exposure was considered an outlier because the total number of BAL cells was 10 times higher than the rest of the group. Therefore the BAL results from this mouse were excluded from the BAL dataset