Fig. 5

Biological reactivity estimate, β_{R CELLS} was derived by averaging the cytotoxic potencies of the particles in A549 and J774A.1 cells, as well as the AhR activity in H1L1.1c2 cells (a). A combined inflammatory estimate, β_{I-V CELLS}, was obtained for each particle by averaging particle inflammatory potency estimates adjusted for cell viability of J774A.1 and A549 cells. The β_{I-V LO} represents the lower estimate (IL-10 and IL-6 potency subtracted from average potency), while β_{I-V HI} is the higher estimate (IL-10 potency subtracted, IL-6 added to average potency) (b). An integrated potency estimate of the particles, Iβ _CELLS was determined by averaging the biological reactivity (cytotoxic potency) and inflammatory estimates for each particle. Iβ _LO represents the lower estimate (IL-10 and IL-6 potency subtracted from average potency). Iβ _HI represents the higher estimate (IL-10 potency subtracted, IL-6 added to average potency) (c)