Fig. 2
From: Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDermâ„¢)
The reconstructed skin micronucleus assay optimised for nanomaterial test articles: (a) Six-well plate containing MatTek Corporation’s 3D epidermis (EpiDermTM) tissue models in trans-well inserts. b Schematic diagram of a single well (cross-section) highlighting the two nanoparticle exposure routes utilised with the 3D models in this study: nanoparticles were either inoculated onto the topical surface or administered into the growth medium. c The developed day-by-day ‘nano-RSMN’ assay protocol from receipt of tissues to harvest detailing dosing, media changes and sequential cyt B regime