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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy

Fig. 4

MCPIP1 induced macrophage apoptosis and fibrosis via autophagy. a Representative western blot showing the effects of RNAi targeting MCPIP1 on the expression of iNOS, Arginase 1 and SOCS3 induced by SiO2 treatment at 50 μg/cm2 for 24 h in U937 cells. b Densitometric analyses of five separate experiments suggested that RNAi targeting MCPIP1 attenuated the SiO2-induced increases in iNOS, Arginase 1 and SOCS3 expression. *p < 0.05 vs the corresponding control group; #p < 0.05 vs the corresponding SiO2 group. c Representative western blot showing the effects of rapamycin and RNAi targeting MCPIP1 on SiO2-induced expression of iNOS, Arginase 1 and SOCS3 in U937 cells. d Densitometric analyses of five separate experiments suggested that rapamycin reversed the effects of RNAi targeting MCPIP1 on SiO2-induced iNOS, Arginase 1 and SOCS3 expression. *p < 0.05 vs the corresponding SiO2 control group; #p < 0.05 vs the corresponding SiO2 + MCPIP1-siRNA group. e Representative western blot showing the effects of RNAi targeting MCPIP1 on SiO2-induced Bax and Bcl-xL expression in U937 cells. f. Densitometric analyses of five separate experiments suggested that RNAi targeting MCPIP1 attenuated the SiO2-induced increase in the Bax/Bcl-xL level. *p < 0.05 vs the control group; # p < 0.05 vs the SiO2 group. g Representative images of Hoechst 33342 staining demonstrating that SiO2-induced U937 cell apoptosis after SiO2 treatment for 24 h at 50 μg/cm2 was attenuated by RNAi targeting MCPIP1. Scale bar = 10 μm. h Representative western blot showing the effects of rapamycin and RNAi targeting MCPIP1 on the SiO2-induced expression of Bax and Bcl-xL in U937 cells. i Densitometric analyses of five separate experiments suggested that rapamycin reversed the effects of RNAi targeting MCPIP1 on the SiO2-induced increase in the Bax/Bcl-xL level. *p < 0.05 vs the SiO2 control group; #p < 0.05 vs the SiO2 + MCPIP1-siRNA group

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