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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy

Fig. 6

SiO2-induced macrophage autophagy was involved in HPF-a cell activation and migration. a Representative images showing the effects of the conditioned media from macrophages on the migration of RFP-labeled HPF-a cells. Scale bar = 80 μm. b Quantification of the scratch width in six separate experiments. *p < 0.05 vs the control group; #p < 0.05 vs the SiO2 group. c Representative western blot showing the effects of the conditioned media from macrophages on the expression of Bax, Bcl-xL, collagen I, collagen III and α–SMA in HPF-a cells. d Densitometric analyses of five separate experiments suggested that pretreatment of U937 macrophages with 3-MA attenuated the decrease in the Bax/Bcl-xL levels and the increases in collagen I, collagen III and α-SMA expression induced by the conditioned media from macrophages treated with SiO2. *p < 0.05 vs the control group; #p < 0.05 vs the SiO2 group. e Representative images of the collagen gel size showing the effects of the conditioned media from macrophages on gel contraction (indicating fibroblast activation)

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