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Table 2 Hematological changes in male rats treated with Cu NPs and Cu MPs following 28 days-repeated oral dose

From: Comparative toxicity and biodistribution assessments in rats following subchronic oral exposure to copper nanoparticles and microparticles

Items Cu NPs (mg/kg/day)
0 100 200 400
No. of rats 10 10 10 10
RBC (1012/L) 7.02 ± 0.234a 6.69 ± 0.388 6.17 ± 0.346** 6.12 ± 0.612**
HB (g/dL) 16.78 ± 1.212 15.77 ± 1.668 12.70 ± 1.628** 11.80 ± 1.187**
HCT (%) 43.43 ± 3.435 42.50 ± 2.432 37.32 ± 3.179** 36.47 ± 5.747**
MCV (fl) 53.73 ± 4.388 50.09 ± 4.618 43.63 ± 9.582** 43.60 ± 4.486**
MCH (pg) 23.47 ± 1.598 21.31 ± 2.788 19.10 ± 3.306** 18.92 ± 2.099**
MCHC (g/dL) 34.68 ± 2.770 34.57 ± 3.317 33.05 ± 4.339 28.31 ± 2.198**
PLT (109/L) 1106.2 ± 104.92 1212.7 ± 125.88 1258.8 ± 166.73* 1262.4 ± 121.65*
RET (%) 2.3 ± 0.23 2.4 ± 0.51 2.7 ± 0.48 6.1 ± 2.17**
WBC (109/L) 11.2 ± 0.95 11.3 ± 2.13 8.5 ± 3.03* 8.3 ± 1.21**
NEU (%) 11.9 ± 0.95 11.2 ± 3.45 23.9 ± 5.41** 36.6 ± 8.15**
LYM (%) 82.3 ± 10.73 82.3 ± 12.79 71.7 ± 13.28 52.7 ± 20.17**
MON (%) 2.2 ± 0.39 2.2 ± 0.33 2.3 ± 0.51 3.0 ± 0.59**
EOS (%) 1.7 ± 0.39 1.7 ± 0.33 1.6 ± 0.53 2.0 ± 0.59
BAS (%) 0.7 ± 0.21 0.8 ± 0.18 0.7 ± 0.27 0.7 ± 0.19
LUC (%) 0.7 ± 0.13 0.7 ± 0.24 1.0 ± 0.19* 1.2 ± 0.25**
Items Cu MPs (mg/kg/day)
  0 100 200 400
No. of rats 10 10 10 10
RBC (1012/L) 7.04 ± 0.312 7.20 ± 0.286 7.00 ± 0.403 6.96 ± 0.388
HB (g/dL) 16.65 ± 1.326 16.72 ± 1.169 15.96 ± 1.563 16.12 ± 1.971
HCT (%) 43.79 ± 4.793 43.61 ± 4.201 40.16 ± 5.776 41.63 ± 3.304
MCV (fl) 53.81 ± 5.716 52.70 ± 6.621 52.63 ± 4.184 52.34 ± 7.206
MCH (pg) 23.61 ± 3.392 24.32 ± 2.562 24.52 ± 1.397 22.93 ± 4.257
MCHC (g/dL) 34.71 ± 3.392 29.67 ± 5.561 32.09 ± 3.397 32.25 ± 4.257
PLT (109/L) 1179.7 ± 202.53 1047.4 ± 218.36 1259.8 ± 187.54 1236.5 ± 232.71
RET (%) 2.5 ± 0.58 2.6 ± 0.37 2.4 ± 0.39 2.7 ± 0.44
WBC (109/L) 11.3 ± 1.49 11.9 ± 2.48 12.3 ± 3.49 11.56 ± 2.23
NEU (%) 12.2 ± 2.23 10.8 ± 3.91 13.2 ± 4.45 11.9 ± 3.71
LYM (%) 82.9 ± 13.2 85.0 ± 22.47 82.7 ± 16.66 83.5 ± 13.09
MON (%) 2.2 ± 0.48 2.3 ± 0.31 2.5 ± 0.43 2.3 ± 0.54
EOS (%) 1.6 ± 0.42 1.5 ± 0.24 1.6 ± 0.40 1.8 ± 0.48
BAS (%) 0.7 ± 0.23 0.7 ± 0.17 0.8 ± 0.13 0.7 ± 0.21
LUC (%) 0.7 ± 0.22 0.9 ± 0.29 0.9 ± 0.29 0.9 ± 0.23
  1. Note. RBC red blood cell, HB hemoglobin, HCT hematocrit, MCV mean corpuscular volume, MCH mean corpuscular hemoglobin, MCHC mean corpuscular hemoglobin concentration, PLT platelet, RET reticulocytes, NEU neutrophils, LYM lymphocytes, MON monocytes, EOS eosinophils, BAS basophils, LUC large unstained cells
  2. aValues are presented as mean ± SD
  3. *, ** P < 0.05, P < 0.01 versus vehicle control group