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Fig. 9 | Particle and Fibre Toxicology

Fig. 9

From: Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions

Fig. 9

Functionality, integrity and intracellular ROS levels of cells exposed in submerged conditions in plates. Co-cultures (A549 + THP-1) were exposed in plates for 24 h to suspensions of TiO2 (NM105, NM101, NM100) and CeO2 (NM212), to achieve deposited doses of around 1, 3 and 10 and 20 μg/cm2. Alamar blue® and LDH assays were performed to assess functionality and integrity of the cells, respectively. A DCF assay was performed to measure intracellular ROS levels and H2O2(1 mM) was used as positive control for the assay (not shown on the graph). Data represent the mean ± SD of three independent experiments. A Kruskal-Wallis test followed by Dunn’s post-hoc test were performed to compare treated groups to controls (*p < 0.05; **p < 0.01; ***p < 0.001)

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