Skip to main content
Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle

Fig. 4

Expression level of glial fibrillary acidic protein (GFAP) in the cerebral cortices of offspring. a, b Quantification of GFAP protein expression in the cerebral cortices of offspring by Western blotting. A one-way ANOVA showed a significant effect of CB-NP treatment (F3, 16 = 17.46, p < 0.001). Post hoc Dunnett tests showed that GFAP protein expression was significantly (**p < 0.01) increased in the middle-dose and high-dose groups. c-m) Light micrographs of GFAP-positive astrocytes in the cerebral cortices of offspring. Scale bars represent 100 μm (c, e, h, k), 50 μm (d, f, i, l), and 10 μm (g, j, m), respectively. Cortices from 6-week-old male mice in the high-dose (k-m), middle-dose (g-j), and low-dose (e, f) groups (e, f) and control group (c, d) are shown. d, f, i, l) Enlarged views of panels c, e, h, and k. j, m Enlarged views of panels i and l. g Enlarged view of panel j. Arrows indicate enlarged PAS-positive granules. Data are presented as the mean ± standard deviation. Abbreviations: GFAP, glial fibrillary acidic protein; NC, negative control; Olf, olfactory bulb; Cx, cerebral cortex; cc, corpus callosum; Str, striatum

Back to article page