Fig. 5
Expression level of aquaporin-4 (Aqp4) protein in the cerebral cortices of offspring. a, b Quantification of Aqp4 protein in the cerebral cortices of offspring by western blotting. A one-way ANOVA showed a significant effect of CB-NP treatment (F3, 16 = 6.95, p < 0.01). Post hoc Dunnett tests showed that Aqp4 protein expression was significantly (*p < 0.05) increased in the high-dose group. c-k Fluorescent micrographs of Aqp4 and glial fibrillary acidic protein (GFAP) expression in the cerebral cortices of 6-week-old offspring and mice of a more advanced age (60-week-old). All scale bars represent 80 μm. The cerebral cortices of 6-week-old male mice from the control (c-e) and high-dose (f-h) groups are shown. i Cerebral cortices of 60-week-old male mice as a positive control. c, f Aqp4; d, g GFAP; e, h, i merge of Aqp4 (red), GFAP (green), and nuclear stain (blue). j, k Quantification of Aqp4 immunofluorescence in linear regions placed over cerebral blood vessels (orange line on c and light blue line on f). *p < 0.05, unpaired t-test. Data are presented as the mean ± SD. Abbreviations: Aqp4, aquaporin-4; GFAP, glial fibrillary acidic protein; NC, negative control