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Table 4 Significantly enriched gene ontology categories according to cerebral cortex microarray data

From: Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle

Gene ontology Enrichment factor P-value Gene symbol up-regulation Gene symbol down-regulation
Angiogenesis 3.66 <0.001 Flt1, Kdr, Nos3, Ptprb, Robo4, Sox17, Tbx1, Tgfa, Tie1  
Positive regulation of mesenchymal cell proliferation 10.11 <0.001 Foxf1, Foxp2, Kdr, Tbx1  
positive regulation of epithelial cell proliferation 6.64 <0.001 Erbb2, Foxp2, Kdr, Tbx1, Tgfa  
Growth factor binding 9.5 <0.001 Cyr61, Erbb2, Flt1, Kdr  
Patterning of blood vessels 8.47 0.001 Cxcl12, Flt1, Lef1, Tbx1  
Ventricular septum development 14.7 0.001 Cyr61, Luzp1, Stra6  
Chemotaxis 4.56 0.005 Ccr3, Cmtm2b, Cxcl12, Cyr61, Flt1  
Positive regulation of endothelial cell proliferation 5.7 0.005 Ccr3, Cxcl12, Kdr, Rptor  
Vasculogenesis 5.7 0.005 Foxf1, Kdr, Sox17, Tie1  
Blood vessel development 5.41 0.006 Foxf1, Stra6, Tbx1, Tie1  
Positive regulation of cell-substrate adhesion 7.13 0.008 Cyr61, Foxf1, Nid1  
Positive regulation of cell migration 3.36 0.009 Cxcl12, Cyr61, Kdr, Lef1, Flt1, Foxf1  
  1. Expression levels of 268 mRNAs were altered in a dose-dependent fashion in the high dose group versus the control group. Functional features of these 268 mRNAs were extracted using gene annotation by gene ontology (enrichment factor ≥ 2, p value ≤ 0.01, symbol number ≥ 3). Enrichment factors for each Gene ontology were defined as (nf/n)/(Nf/N), where nf is the number of flagged (differentially expressed) genes within a given category, Nf is the total number of genes within that same category, n is the number of flagged genes on the entire microarray, and N is the total number of genes on the microarray