Fig. 7
Flow cytometric analysis of leukocytes in the draining lymph nodes of mice. Mice were treated with QDs 0, 4, or 7 days post 180 mJ/cm2 UVB radiation exposure for 24 h and the lymph nodes were processed for analysis. Cells positive for the QD605 marker are co-localized with an intact QD. Leukocytes were sorted into general antigen presenting cells (MHCII+) (a), Langerhans cells (CD207+) (b), monocytes (CD11b+) (c), and macrophages (F4/80+) (d). The total, isolated leukocyte populations are represented by the grey bars, and the isolated leukocyte/QD605+ double positive cells are represented by the red squares. The total events collected in each group were 2 × 106. The graphs represent the mean +/- standard error, N = 3. The statistics are based on one-way ANOVA with post hoc Tukey tests, except for the data with unequal variance (F4/80 + QD605+, CD11b+) for which a Kruskal-Wallis test was used (*p < 0.05)