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Fig. 7 | Particle and Fibre Toxicology

Fig. 7

From: Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairment

Fig. 7

Representative flow cytograms for cell cycle analysis (a), annexin V/PI assay (b) and their statistical analysis (c) depicting increase in % apoptotic cells upon GCNF exposure. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. (d) TEM photomicrographs depicting the nuclear fragmentation (white arrow), swollen mitochondria (red arrow) and chromatin condensation (yellow arrow) which are the hall mark of apoptosis. Including this, particle accumulation (green arrow) was also found. Immunoblotting analysis of various apoptotic protein in dose dependent manner (e) as well as in presence of Q-Vd-OPh (caspase inhibitor) (f). GAPDH was used as a loading control. Densitometry analysis is represented by the digits given above the respective blots and also provided in Additional file 4: Figure S4a, S4b. (g) Representative flow cytograms and corresponding statistical analysis for altered mitochondrial membrane potential. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. Representative TEM photomicrographs (h) and corresponding fluorescence images (i) of A549 transfected with Mito - DsRed Plasmid showing the damage to mitochondria. In TEM, green arrow – healthy mitochondria with proper cristae whereas and yellow arrow – swollen mitochondria with dissolved cristae structure. Scale Bar – 20 μm. Per view 25 cells and 4 views per group were analyzed

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Particle and Fibre Toxicology

ISSN: 1743-8977

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