|  | Absolute cell numbers | Percentage of cell type in sample (%) | ||||||
---|---|---|---|---|---|---|---|---|---|
Dose level of MWCNT |  Control | 2 μg | 18 μg | 67 μg |  Control |  2 μg | 18 μg | 67 μg | |
4 wks | N | 4 | 9 | 9 | 6 | 4 | 9 | 9 | 6 |
 | Neutrophils (×103) | 7.8 ± 2.9 | 13.1 ± 7.6 | 272 ± 168 | 1173 ± 318b | 1.1 ± 0.5 | 1.7 ± 0.9 | 10.9 ± 3.7 | 16.2 ± 3.3a |
Macrophages (×103) | 785 ± 190 | 607 ± 46 | 1073 ± 219 | 4468 ± 662c | 83.1 ± 2.7 | 75.7 ± 2.8 | 76.9 ± 3.7 | 66.8 ± 4.6 | |
Eosinophils (×103) | 25.2 ± 12 | 54.5 ± 40 | 5.7 ± 2.4 | 106 ± 34 | 3.9 ± 2.3 | 4.3 ± 2.8 | 0.3 ± 0.1 | 1.4 ± 0.4 | |
Lymphocytes (×103) | 0.7 ± 0.7 | 2.0 ± 1.5 | 6.8 ± 3.4 | 103 ± 52 | 0.1 ± 0.1 | 0.2 ± 0.1 | 0.7 ± 0.3 | 1.4 ± 0.6 | |
Total BAL cells (×103) | 927 ± 195 | 821 ± 80 | 1511 ± 400 | 6855 ± 1095 | - | - | - | - | |
Epithelial cells (×103) | 109 ± 25 | 144 ± 11 | 153 ± 29 | 1013 ± 230c | 11.8 ± 2.0 | 18.2 ± 1.2 | 11.2 ± 2.2 | 14.4 ± 1.9 | |
Dead cells (×103) | 237 ± 53 | 240 ± 23 | 280 ± 47 | 943 ± 115 | - | - | - | - | |
6 wks |  N |  10 | 8  | 6  | 10  |  10 | 8  | 6  | 10  |
 | Neutrophils (×103) | 5.9 ± 3.0 | 5.3 ± 2.2 | 274 ± 118 | 2503 ± 792c | 0.6 ± 0.3 | 0.8 ± 0.3 | 9.3 ± 2.7 | 27.8 ± 5.4c |
Macrophages (×103) | 802 ± 175 | 750 ± 123 | 1867 ± 780 | 4685 ± 1426b | 89.2 ± 1.0 | 94.1 ± 1.1 | 79.5 ± 3.7 | 63.2 ± 4.4c | |
Eosinophils (×103) | 18.8 ± 11 | 1.8 ± 0.7 | 7.1 ± 57 | 15.2 ± 13a | 1.7 ± 0.9 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.1 ± 0.1 | |
Lymphocytes (×103) | 0.3 ± 0.3 | 1.4 ± 1.0 | 26.4 ± 2.5 | 98.5 ± 46 | 0.1 ± 0.1 | 0.2 ± 0.1 | 1.8 ± 0.6b | 1.5 ± 0.5a | |
Total BAL cells (×103) | 898 ± 197 | 793 ± 124 | 2313 ± 942 | 7781 ± 2218b | - | - | - | - | |
Epithelial cells (×103) | 71.6 ± 14 | 34.7 ± 5.4 | 186 ± 55 | 479 ± 137b | 8.6 ± 1.3 | 4.7 ± 0.8 | 9.3 ± 1.7a | 7.4 ± 1.1 | |
Dead cells (×103) | 121 ± 43 | 121 ± 35 | 281 ± 131 | 840 ± 186c | - | - | - | - | |
8 wks | N |  12 | 3 | 3 | 13  |  12 | 3 | 3 | 13  |
 | Neutrophils (×103) | 10.1 ± 3.8 | 0.0 ± 0.0 | 400 ± 169 | 986 ± 261b | 1.4 ± 0.5 | 0.0 ± 0.0 | 20.3 ± 4.5 | 22.8 ± 4.2c |
Macrophages (×103) | 633 ± 89 | 458 ± 33 | 1453 ± 678 | 2346 ± 470b | 82.7 ± 2.0 | 85.5 ± 9.0 | 73.2 ± 2.8 | 63.8 ± 3.6c | |
Eosinophils (×103) | 2.8 ± 1.6 | 49.8 ± 48 | 10.6 ± 5.7 | 25.6 ± 12 | 0.4 ± 0.2 | 8.5 ± 8.3a | 0.7 ± 0.4 | 0.8 ± 0.3 | |
Lymphocytes (×103) | 0.7 ± 0.8 | 1.8 ± 0.9 | 35.2 ± 31 | 49.6 ± 1.4b | 0.1 ± 0.1 | 0.3 ± 0.2 | 1.2 ± 0.7 | 1.5 ± 0.5a | |
Total BAL cells (×103) | 784 ± 130 | 540 ± 153 | 2013 ± 945 | 3830 ± 733b | - | - | - | - | |
Epithelial cells (×103) | 137 ± 40 | 30.9 ± 5.1 | 113 ± 8.0 | 422 ± 108 | 15.4 ± 1.9 | 5.7 ± 0.7a | 4.7 ± 1.5a | 11.0 ± 1.3 | |
Dead cells (×103) | 174 ± 59 | 43.7 ± 29 | 251 ± 63 | 479 ± 93 | - | - | - | - |