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Fig. 1 | Particle and Fibre Toxicology

Fig. 1

From: Osteopontin enhances multi-walled carbon nanotube-triggered lung fibrosis by promoting TGF-β1 activation and myofibroblast differentiation

Fig. 1

Induction of OPN by MWCNTs. WT mice received DM or MWCNTs (40 μg) and were sacrificed on days 1, 3, 7, 14, 28, and 56 post-exposure. (a) qRT-PCR analysis of Opn mRNA in total RNA extracts from lung tissues (n = 4). Gapdh gene was used as the internal control. (b) Immunoblotting of OPN in lung tissue whole protein lysates (n = 2). Lung proteins from randomly selected samples of each group were studied, and a representative blotting image is presented. OPN protein with post-translational modifications was detected. Actin protein was used as the loading control. (c) Immunohistochemistry of OPN on lung tissue sections. Red indicates positive staining of OPN, and blue indicates nuclear counterstaining (scale bar: 20 μm)

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Particle and Fibre Toxicology

ISSN: 1743-8977

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