Fig. 10

Induction of OPN and TGF-β1 expression and activation by MWCNTs in cultured lung fibroblasts. In (a) and (c), cells were treated with DM or 5 μg/ml MWCNTs for 24 h. OPN expression (a, red) and TGF-β1 expression (c, green) were determined by immunofluorescence. Blue indicates nuclear staining (scale bar: 20 μm). In (b) and (d), cells were treated with DM, 2 μg/ml MWCNTs, or 5 μg/ml MWCNTs for 24 h. Levels of OPN with post-translational modifications (b) and active TGF-β1 (d) were detected by immunoblotting (n = 2). Actin was used as the loading control