Fig. 8From: Osteopontin enhances multi-walled carbon nanotube-triggered lung fibrosis by promoting TGF-β1 activation and myofibroblast differentiationActivation of TGF-β1 signaling in the lungs. WT and Opn KO mice were exposed to DM or 40 μg MWCNTs for 7 or 28 days. (a) Immunohistochemistry of p-Smad2/3 on lung tissue sections. Red indicates positive staining of p-Smad2/3 (scale bar: 20 μm). Relative intensity of positive staining was measured and presented as mean ± SD (n = 4). (b) Immunoblotting of p-Smad2/3 and Smad2/3 (n = 2). On the blot for p-Smad2/3, the upper band is p-Smad2 and the lower band is p-Smad3; and on the blot for Smad2/3, the upper band is Smad2 and the lower band is Smad3. Actin was used as the loading controlBack to article page