Fig. 4

Characterization of peripheral blood monocytes and leucocytes for surface expression of adhesion molecules following inhalation and intra-arterial infusion of carbon nanoparticle (CNP) in mice compared to control using fluorescent automated cell sorting (FACS). a. Reduced expression of CD49d and CD11b was noted in granulocytes after 24 h of CNP inhalation exposure whereas CD18 expression was reduced in granulocytes at both 4 h and 24 h post inhalation exposure. b. Reduced expression of CD49d in monocytes was observed after 24 h of CNP inhalation exposure, while reduced CD18 expression in monocytes was noted following both 4 h and 24 h post CNP inhalation exposure. c. No altered expression of CD49d, CD11b and CD18 was noted in granulocytes following 4 h of intra-arterial infusion of CNP. d. No altered expression of CD49d, CD11b and CD18 was noted in monocytes following 4 h of intra-arterial infusion of CNP. Data are shown as Mean ± SEM; inhalation: n = 8, infusion: n = 6; and asterisks (*) denote p < 0.05. White bars: Clean air exposed; Gray bars: CNP exposed