Fig. 5

Maturation markers on steady-state DCs. Immature DCs were incubated for 18 h at 37 °C with the indicated stimulus and their surface markers were analyzed by flow cytometry. a Increased CD69 on the surface of DCs exposed to SAS particles (13-nm primary diameter) or oligonucleotide ODN1668. Plasmacytoid and conventional DCs are the two major subsets differing in B220 expression (numbers denote percentages of events in each gate). b Representative histograms showing dose-dependent changes of CD69 and CD40 on conventional DCs exposed to the indicated stimuli. Control, unstimulated DCs in culture medium. c Representative histograms showing dose-dependent changes in the display by CD69, CD40, CD86 and CD62L on plasmacytoid DCs. d Quantification of maturation markers by median fluorescence intensity (MFI) on plasmacytoid DCs exposed to SAS particles (13-nm primary size). Statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) was determined by one-way ANOVA with Dunnet’s correction, n = 3 experiments with independent bone marrow isolates; error bars, s.e.m