Fig. 7From: Prenatal tobacco smoke exposure predisposes offspring mice to exacerbated allergic airway inflammation associated with altered innate effector functionPrenatal ETS causes a reduction in pulmonary NK cell numbers in juvenile and adult mice. The effect of exposure to prenatal ETS or filtered air on pulmonary natural killer (NK) cell numbers was examined in both juvenile (7-week old) and adult (18-week old) offspring mice following intranasal challenge with either HDM allergen or PBS (control). After gating on CD3−CD19− cells, the number of CD3−CD19−NK1.1+ NK cells present in the lung mononuclear cells was determined using multicolor flow cytometry and expressed as total number of NK cells (per mouse). Results are mean ± SEM (n = 4), **p < 0.01, ***p < 0.001Back to article page