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Fig. 10 | Particle and Fibre Toxicology

Fig. 10

From: NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM2.5 aspiration

Fig. 10

NF-κB activation regulates miR-574-5p expression in response to PM2.5 exposure. (a) Binding of the NF-κB p65 subunit to the promoter of the miR-574-5p gene. IgG was used as a negative control in the chromatin immunoprecipitation assay. (b1) Knockdown of NF-κB p65 using a shRNA silencing technique eliminates the reduction in miR-574-5p following 10 μg/mL PM2.5 treatment in primary cultured hippocampal neurons. (b2) Knockdown of NF-κB p65 by applying SC-514 (100 μM), an IKKβ specific inhibitor, eliminates the reduction in miR-574-5p following 10 μg/mL PM2.5 treatment in primary cultured hippocampal neurons. (c1) Knockdown of NF-κB p65 using a shRNA reverses the elevation in BACE1 in response to 10 μg/mL PM2.5 treatment in primary cultured hippocampal neurons. (c2) Knockdown of NF-κB p65 by applying SC-514 restores the BACE1 elevation in response to 10 μg/mL PM2.5 treatment in primary cultured hippocampal neurons. Green fluorescent protein (GFP) expression was used as a marker to indicate viral infection in the immunoblot assay. The data are expressed as the means ± SE (n = 4 to 8). */# P < 0.05; **/## P < 0.01; ***/### P < 0.001

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