Fig. 7

Direct inhibition of BACE1 expression by miR-574-5p was detected with a dual-luciferase-luciferase reporter system. BACE1 contains a conserved 3′-UTR sequence (positions 1458–1464) that perfectly complements the miR-574-5p seed sequence (both are shown in blue). The mutant BACE1 3′-UTR contains mutations in the miR-574-5p binding site that disrupt base pairing (indicated in red). The data regarding the different effects of miR-574-5p on the BACE1 3′-UTR and its mutant are presented as the means ± SE (n = 6). */^# P < 0.05; **/^## P < 0.01. NC = negative control