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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Protein corona: implications for nanoparticle interactions with pulmonary cells

Fig. 2

Analysis of NP-bound proteins by 1D gel electrophoresis and mass spectrometry. a The molecular weights (kDa) of reference proteins are shown in lane MW. A representative gel from one experiment is shown. NP-corona components identified by LC-MS are indicated on right. b LC-MS profiles of the most abundant BAL proteins and influence of NP type on corona profile. The nine most abundant proteins are shown. The total proteins per mg NPs were in the order of CeO2 = Si-CeO2 = BaSO4 < ZnO. (* P < 0.05, significant variations in each protein among 4 NP types). c When expressed as % of total bound protein, transferrin, albumin and α-1-antitrypsin were greater in the corona of ZnO and BaSO4 than of both CeO2 NPs. (* P < 0.05, significant variations in each protein among 4 NP types). d When expressed as bound proteins per surface area of NPs, transferrin and α-1-antitrypsin were greater in the corona of ZnO than of CeO2 NPs. Bound albumin was greater in the coronas of ZnO and BaSO4 than of both CeO2 NPs. Data are mean ± SD, n = 3 rats per NP)

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