Fig. 1

SiNPs inhibit TRPV4 activation in 16HBE cells. a, b, c, d Effects of the TRPV4 agonist GSK1016790A on the [Ca²⁺]_i in the absence (a, n = 333) or in the presence of SiNPs 10 μg/ml (b, n = 234), 100 μg/ml (c, n = 163) and 1000 μg/ml (d, n = 114). The thick continuous traces correspond to the average responses and the thin dashed traces correspond to the mean plus/minus the standard errors. The dashed lines and black arrows indicate the amplitude of the average Ca²⁺ responses recorded after 2 min application of GSK1016790A, with respect to the immediate previous basal [Ca²⁺]_i. The gray arrows point to the peak of basal Ca²⁺ responses to SiNPs. e Concentration dependence of the maximal amplitude of basal Ca²⁺ responses induced by the application of SiNPs. f Average amplitude of responses to 10 nM GSK1016790A when applied in the presence of SiNPs at different concentrations. In (e) and (f) the solid lines represent fits with Hill functions (see Methods)