Fig. 5
From: Silica nanoparticles inhibit the cation channel TRPV4 in airway epithelial cells
SiNPs inhibit activation of mouse TRPV4 heterologously expressed in HEK293T cells. a, b Effects of the TRPV4 agonist GSK1016790A on the [Ca2+]i in the absence (a, n = 127) or in the presence of 10 μg/ml SiNPs (b, n = 243). The thick continuous traces correspond to the average responses and the thin dashed traces correspond to the mean plus/minus the standard errors. The black and grey traces correspond to cells with high or low basal [Ca2+]i, respectively (see text). c Concentration-dependent effects on SiNPs on the intracellular Ca2+ levels in cells with low and high basal Ca2+. d Average amplitude of responses to 10 nM GSK1016790A when applied in the presence of SiNPs at different concentrations. The solid line represents a fit with a Hill function (see Methods). e, f Effects of the TRPV4 agonist 4αPDD on the [Ca2+]i in the absence (a, n = 347) or in the presence of SiNPs (b, n = 164). The traces are color-coded as in panels (a) and (b)