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Table 2 Instruments and conditions used for homogenization, acid treatment, and analysis

From: Kinetics and dissolution of intratracheally administered nickel oxide nanomaterials in rats

Homogenate
 Volume of pure water for homogenate Liver 10 mL
Other organs 2 mL
Face 2 mL
Food 2 mL
Gastrointestinal contents 2 mL
 Electric homogenizer PT10–35 (Kinematica AG, NS-50, and NS-52; Microtec Co. Ltd., Chiba, Japan)  
Acid treatment for NiO A and B
 Volume of samples and acid Homogenate sample 1.0 g
HNO3 (68%) 1.0 mL
 Ashing 180 °C (2 h)  350 °C (2 h) 550 °C (3 h)  
 Electric furnace TFF45-C (Tokyo Technological Labo Co. Ltd., Kanagawa, Japan)  
Acid treatment for NiO C and D
 Volume of samples and acid Homogenate sample 1.0 g
HNO3 (68%) 0.5 mL
H2O2 0.2 mL
 Heating 180 °C (20 min)  
 Microwave sample preparation instrument ETHOS 1 (Milestone Srl, Sorisole, Italy) or Speedwave 4 (Berghof, Eningen, Germany)  
Analysis
 ICP-MS ELEMENT 2/ ELEMENT XR (Thermo Fisher Scientific, Waltham, MA, USA)  
 RF output 1200 W  
 Target mass (m/z) 60Ni (mass: 59.9308)