Table 2 Instruments and conditions used for homogenization, acid treatment, and analysis
From: Kinetics and dissolution of intratracheally administered nickel oxide nanomaterials in rats
Homogenate | ||
Volume of pure water for homogenate | Liver | 10 mL |
Other organs | 2 mL | |
Face | 2 mL | |
Food | 2 mL | |
Gastrointestinal contents | 2 mL | |
Electric homogenizer | PT10–35 (Kinematica AG, NS-50, and NS-52; Microtec Co. Ltd., Chiba, Japan) | |
Acid treatment for NiO A and B | ||
Volume of samples and acid | Homogenate sample | 1.0 g |
HNO3 (68%) | 1.0 mL | |
Ashing | 180 °C (2 h) ⇒ 350 °C (2 h) ⇒ 550 °C (3 h) | |
Electric furnace | TFF45-C (Tokyo Technological Labo Co. Ltd., Kanagawa, Japan) | |
Acid treatment for NiO C and D | ||
Volume of samples and acid | Homogenate sample | 1.0 g |
HNO3 (68%) | 0.5 mL | |
H2O2 | 0.2 mL | |
Heating | 180 °C (20 min) | |
Microwave sample preparation instrument | ETHOS 1 (Milestone Srl, Sorisole, Italy) or Speedwave 4 (Berghof, Eningen, Germany) | |
Analysis | ||
ICP-MS | ELEMENT 2/ ELEMENT XR (Thermo Fisher Scientific, Waltham, MA, USA) | |
RF output | 1200 W | |
Target mass (m/z) | 60Ni (mass: 59.9308) | |