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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Sub-chronic inhalation of lead oxide nanoparticles revealed their broad distribution and tissue-specific subcellular localization in target organs

Fig. 5

Effect of inhalation of lead oxide nanoparticles on liver following 6 weeks exposure. a, e Hematoxylin-Eosin stained liver samples. b, f Toluidine Blue stained semithin sections. c, g Green-Trichrome stained liver samples. d, h PAS staining. Arrows show alterations in liver tissue as hypertrophy of hepatocytes with karyomegaly, vacuolated cytoplasm (e, f), and focal necrosis (fn). Detail of groups of lipid droplets in hepatocyte identifiably in Toluidine Blue staining (the upper corner in panel f). Scale bar in panels a-h = 100 μm. i-p Subcellular changes in liver tissue after lead treatment. i, j affected hepatocytes (hc) with vacuolated cytoplasm, unnatural sinusoidal endothelial cells (si), and bile duct epithelial cells (bd). k, l abundant lipid droplets (li) and nuclei (nu) in hepatocyte. m-o nanoparticles (arrowheads) in hepatocyte mitochondria (mi), next to nucleus (nu). p nanoparticles (arrowhead) in hepatocyte peroxisome (pe)

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