Primary genotoxicity in the liver following pulmonary exposure to carbon black nanoparticles in mice

Table 2 BAL fluid cellular composition following intratracheal instillation of 162 μg of TiO₂, CeO₂ or CB NPs 1, 28 and 180 days post-exposure

BAL fluid cellular composition after day 1
	Control	TiO₂	CeO₂	CB
Total cells (× 10⁵)	0.9 ± 0.6	2.5 ± 0.9**	3.4 ± 1.1***	2.4 ± 1.0**
Neutrophils (× 10⁴)	0.2 ± 0.2	20.2 ± 8.5***	26.1 ± 9.5***	20.1 ± 8.3***
Macrophages (× 10⁴)	9.0 ± 5.7	4.5 ± 1.9	6.1 ± 1.5	2.9 ± 1.5**
Lymphocytes (× 10²)	1.9 ± 2.9	0.0 ± 0.0	7.1 ± 7.9	0.0 ± 0.0
Eosinophils (×10³)	0.0 ± 0.1	3.0 ± 2.5**	13.3 ± 9.2***	7.6 ± 6.8**
BAL fluid cellular composition after day 28
	Control	TiO₂	CeO₂	CB
Total cells (× 10⁵)	1.4 ± 0.5	1.1 ± 0.2	1.6 ± 0.7	1.6 ± 0.8
Neutrophils (×10⁴)	0.6 ± 0.3	1.9 ± 0.9*	2.6 ± 1.6**	3.8 ± 1.7***
Macrophages (×10⁴)	7.9 ± 2.0	9.3 ± 2.3	12.9 ± 5.5	11.9 ± 5.5
Lymphocytes (×10²)	8.5 ± 13.7	35.5 ± 44.3	28.3 ± 55.4	53.7 ± 78.1
Eosinophils (×10³)	51.2 ± 39.3	0.1 ± 0.2**	0.2 ± 0.3*	0.0 ± 0.0***
BAL fluid cellular composition after day 180
	Control	TiO₂	CeO₂	CB
Total cells (× 10⁵)	0.7 ± 0.3	1.0 ± 0.2	0.9 ± 0.3	1.3 ± 0.6
Neutrophils (×10⁴)	0.3 ± 0.4	0.6 ± 0.4	0.3 ± 0.3	1.1 ± 0.7
Macrophages (×10⁴)	7.0 ± 2.9	9.4 ± 2.1	8.4 ± 2.9	11.9 ± 4.9
Lymphocytes (×10²)	0.0 ± 0.0	0.0 ± 0.0	0.5 ± 1.1	0.0 ± 0.0
Eosinophils (×10³)	0.3 ± 0.4	0.4 ± 0.5	4.9 ± 6.8	4.9 ± 4.4

Particle exposed groups n = 6, vehicle control n = 6. All values are presented as mean ± SD. An asterisk (*) denotes P ≤ 0.05, (**) P ≤ 0.01, (***) P ≤ 0.001 of statistically significantly different cells level in exposed groups versus vehicle control

ISSN: 1743-8977