Fig. 1

Evaluating DNA fragmentation and read quality for chromatin immunoprecipitation (ChIP) sequencing. a Using gel electrophoresis, DNA fragments were evaluated to determine size and distribution (average size of fragments = 654.3 bp). Two controls and two maternal nano-TiO₂exposed representative samples are shown. Sample quality was assessed using FastQC for both forward and reverse reads (b) before and (c) after using Trimmomatic. Con = control, Exp = maternal nano-TiO₂ exposed, H3K4me3 and K4 = histone 3 lysine 4 tri-methylation, K27 = histone 3 lysine 27 tri-methylation